Non-integrative microfluidic technology to generate hiPSCs from patient samples.
Mission
The provides a specialized service for reprogramming somatic cells into high-quality human induced pluripotent stem cells (hiPSCs). We bridge the gap between complex cell engineering and accessible research tools.
From biological sample to hiPSC delivery in 5 steps.
Biopsy or derived fibroblast collection as starting material.
Somatic cells are reprogrammed into a pluripotency state using non-integrative mRNA on a patented microfluidic platform.
High-fidelity hiPSCs verified via protein-level pluripotency profiling and rigorous genomic integrity checks.
Standardized hiPSC expansion and secure storage in liquid nitrogen vapour.
Certified storage and distribution of hiPSC lines, ensuring full ethical alignment and formal regulatory compliance.
Technological platforM
Our method leverages advanced microfluidic technology to optimize mRNA delivery.
By choosing an mRNA-based approach, we offer the following key advantages:
Reprogramming is based on sequential transfections of short-lived mRNA encoding for Yamanaka factors:
POU5F1, SOX2, KLF4, C-MYC, NANOG and LIN28.
Exogenous mRNA has a cellular lifetime of ~24h, generating hiPSC colonies free of exogenous RNA.
Improved mRNA delivery
Fast accumulation of endogenous cellular factors (positive feedback)
Low starting cell number required
High success rate: simultaneus reprogramming of up to 10 cell Lines simultaneusly
Invented by our team of specialists (Gagliano O, Luni C, Qin W, Bertin E, Torchio E, Galvanin S, Urciuolo A, Elvassore N). Published in Nature Protocols (2019) and Nature Cell Biology (2019). Enables cost-effective cellular reprogramming services.
OUTCOME
Custom establishment and expansion of donor-derived primary cell lines tailored to project specifications.
A seamless somatic-to-hiPSC transition utilizing our optimized reprogramming protocols to ensure high-quality outcomes.
Management of a robust hiPSC repository, ensuring secure storage and worldwide access to well-characterized lines for the scientific community
Standardized assays to evaluate and validate the multilineage differentiation potential of hiPSC lines.
Specialized hands-on training in microfluidic-driven mRNA delivery, enabling laboratories to implement high-efficiency reprogramming in-house.
About
Liliana Contu
About
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Una nuova risorsa dedicata alla ricerca biomedica prende vita presso il VIMM – Istituto Veneto di Medicina Molecolare. È stata...
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Accepted material types:
Skin biopsy
Liquid biopsy
Fibroblasts at passage lower than 7
Quality requirements:
Mycoplasma-free material
Tested for contagious pathogens (specify X, Y, Z)
